UC知道求 英语达人帮我翻译一点东西 （不要在线翻译软件翻译）
来源：百度知道 编辑：UC知道 时间：2024/07/04 03:09:46
试验材料及方法简述如下：
植物材料为东农48、东农47、东农46、东农44和东农42，微生物材料为含质粒pSPUD5（内含cryⅤ抗虫基因，即目的基因）的大肠杆菌DH5α。
首先，培养大肠杆菌，令其大量繁殖；然后用碱裂解法收集质粒pSPUD5，并将其纯化，备用。在大豆盛花期挑选符合要求的花，将准备好的质粒pSPUD5导入，做好标记；并定期观察；秋后，按组合收获导入一代（D1）的种子，在收获袋上记录好标记牌上的记录，统计结荚率、每荚粒数和收获总量。
结果与分析及结论简述如下：
共导入2 760朵花，获得1 008个荚和2 322粒种子。受体为东农48、东农47、东农46、东农44和东农42。
1． 各品种成荚率相差较大，成荚率最高的东农42比最低的东农44高出近30个百分点，说明成荚率受大豆基因型控制。
2． 在上午选取花冠高于花萼2-3mm且花色新鲜的花朵进行转化效果较好，结实率与转化率都高。
3． DNA浓度要恰当，且纯度要高，本研究测定的最佳DNA浓度为300ng/ml。
求达人帮忙翻译下 谢谢
The purpose of this study and its significance is the cry Ⅴ insect-resistant genes and to the whole soybean genes to increase the resistance of soybean.
Test materials and methods outlined as follows:
East plant material for agricultural 48, East 47 farmers, agricultural East 46, East 44 farmers and 42 agricultural East, microbial materials containing plasmid pSPUD5 (containing cry Ⅴ insect-resistant gene, the gene that is) of E. coli DH5 α.
First of all, E. coli cultivation, breeding them a lot and then use alkaline lysis method to collect plasmid pSPUD5, and purification, spare. Sheng soybean flowering period in selected to meet the requirements of flowers, will be prepared plasmid pSPUD5 import and do a good job tags, and regular observation; settle, according to portfolio into harvest generation (D1) of the seed, recorded on the bag in a good harvest tag On the licensing records, statistics pod rate of grains per pod and the total harvest.
Results and ana