求助生物学论文摘要的英文翻译~~谢谢！拜托高手帮我翻译一下

来源：百度知道 编辑：UC知道 时间：2024/07/02 16:56:02
目的：了解甲状腺激素受体β△基因在大鼠不同组织中的分布特点，熟悉用PT-PCR分析结果。方法：TRβ△基因在多种组织中均有表达，其中肝脏、脑、骨胳肌、心脏、肾脏表达最多。提取大鼠多处组织的mRNA, 以提取的mRNA为模板逆转录合成cDNA第一链。并以之为模板PCR扩增TRβ△双链cDNA。根据转录出的目的基因设计两条引物。采用荧光定量PCR的方法进行定量分析。结果：提取组织总RNA，OD260/OD280均介于1.7-2.0之间，OD260/230介于1.5-1.9之间。琼脂糖凝胶电泳鉴定PCR扩增结果显示,在预期的约160bp左右有目的扩增产物带。TRβΔ PCR产物的琼脂糖凝胶电泳鉴定结果表明，经PCR扩增出的DNA分子量大小为176bp的目的条带。 目的基因及管家基因定量测定，其扩增曲线和溶解曲线未见杂峰，同时融解曲主峰对应的Tm值与扩增产物的理论Tm相近。结论：研究结果显示TRβ△基因于mRNA水平的表达有一定的空间分布特点：肝，脑，脾，肾组织中均有该基因表达，且脾中的表达量明显高于其他组织。

Objective: To β △ thyroid hormone receptor gene in rats in the distribution of different characteristics, and is familiar with using PT-PCR analysis of the results. Method: TR β △ gene were expressed in a variety of organizations, including the liver, brain, skeletal muscle, heart, kidneys express the most. From various organizations in the mRNA, to extract the mRNA as a template retroviral cDNA synthesis of the first chain. And the template for PCR amplification TR β △ double-stranded cDNA. According to the purpose of gene transcription design of the two primers. Using fluorescence quantitative PCR method of quantitative analysis. Results: The Organization of extracting RNA, OD260/OD280 are between 1.7-2.0 between, OD260/230 range between 1.5-1.9. Identification of agarose gel electrophoresis of PCR results showed that in the expected around about 160 bp PCR products with a purpose. TR βΔ PCR product of the identification of agarose gel electrophoresis results showed that the PCR a