急求论文的中文摘要的英文翻译~~~谢谢好心人
来源:百度知道 编辑:UC知道 时间:2024/09/21 05:45:49
Solves the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic, the understanding real-time fluorescence quota PCR principle and the application method. Uses real-time fluorescence quota PCR familiar the method analysis result. Method: TRβ△ the gene has the expression in many kinds of organizations, the liver, the brain, the skeleton muscle, the heart, the kidney expression are most. Withdraws the big mouse liver, the brain, the skeleton muscle, the heart, the kidney and so on many place organization's mRNA, withdraws mRNA is the template counter duplication synthesizes the cDNA first chain. And increases double strand cDNA take it as template PCR. According to the goal gene which copies designs two to direct the thing. Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis. Finally: 1. the extraction organizes total RNA, OD260/OD280 to be situated between 1.7-2.0, OD260/230 is situated between 1.5-1.9. After