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Chitosan-based scaffolds possess some special properties for use in tissue engineering. First, Chitosan can be formed as interconnected-porous structures by freezing and lyophilizing of chitosan solution or by processes such as an “internal bubbling process (IBP)”where CaCO3 is added to chitosan solutions to generate chitosan–CaCO3 gels in specific shapes by using suitable molds (Chow and Khor, 2000). The interconnectedporous
structure is very important, so that numerous cells can be seeded, migrate into the inside, increase the cell number and should be supplied by sufficient amounts of nutrient. The porous structure of chitosan is a promising characteristic for the development and optimization of a variety of tissue scaffolds and regeneration aids. Regulation of porosity and pore morphology of chitosan-based scaffolds is critical for controlling cellular colonization rates and organization within an engineered tissue. In addition, angiogenesis required for some scaffold applicat

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