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来源:百度知道 编辑:UC知道 时间:2024/06/28 07:42:45
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The tissue is homogenized with chloroform/methanol (2/1) to a final volume 20 times the volume of the tissue sample (1 g in 20 ml of solvent mixture). After dispersion, the whole mixture is agitated during 15-20 min in an orbital shaker at room temperature.
组织样本用氯仿/甲醇(2/1)试剂均质,试剂用量是样本的20倍(1克样本用20毫升的试剂)。离散后,整个混合物在室温下震荡15-20分钟。

The homogenate is either filtrated (funnel with a folded filter paper) or centrifuged to recover the liquid phase.
然后,组织液混合物或者过滤(带滤纸的漏斗)或离心机离心,获得液相。

The solvent is washed with 0.2 volume (4 ml for 20 ml) of water or better 0.9% NaCl solution. After vortexing some seconds, the mixture is centrifuged at low speed (2000 rpm) to separate the two phases. Remove the upper phase by siphoning and kept it to analyze gangliosides or small organic polar molecules.
获得的液体用0.2倍体积的水(20毫升用水4毫升)或者生理盐水冲洗。搅拌几分钟后,混合物低速离心(2000 rpm),出现分层。用吸管吸取上层液相,保留它用于神经脂类或别的小分子极性有机物质。
If necessary (need of removing labelled molecules..

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