UC知道翻译一段实验步骤
来源：百度知道 编辑：UC知道 时间：2024/09/25 14:23:47
不要用软件或在线翻译，那样语句不通顺。
若有满意的，此题一百分。
一小时内。
test procedure
测试程序
carefully follow the recommended washing procedure. do not allow microwells to dry between working steps
认真贯彻建议的洗涤程序。
1.insert a sufficient number of wells into the microwell holder for all standards and samples to be run in duplicate. record standard and sample positions.
2.add 50 ul of each standard solution or prepared sample to separate duplicate wells
3.add 50 ul of the diluted enzyme conjugate to the bottom of each well, mix gently by shaking the plate manually and incubate for 1 h at room temperature(20-25 C/68-77F)
4.pour the liquid out of the wells and tap the microwell holder upside down vigorously (three times in a row) against absorbent paper to ensure complete removal of liquid from the wells.fill all the wells with 250 ul washing buffer(see 10.1.) and pour out the liquid again.repeat two more times.
5.add 100 ul of substrate/chromogen solution to each w
若有满意的，此题一百分。
一小时内。
test procedure
测试程序
carefully follow the recommended washing procedure. do not allow microwells to dry between working steps
认真贯彻建议的洗涤程序。
1.insert a sufficient number of wells into the microwell holder for all standards and samples to be run in duplicate. record standard and sample positions.
2.add 50 ul of each standard solution or prepared sample to separate duplicate wells
3.add 50 ul of the diluted enzyme conjugate to the bottom of each well, mix gently by shaking the plate manually and incubate for 1 h at room temperature(20-25 C/68-77F)
4.pour the liquid out of the wells and tap the microwell holder upside down vigorously (three times in a row) against absorbent paper to ensure complete removal of liquid from the wells.fill all the wells with 250 ul washing buffer(see 10.1.) and pour out the liquid again.repeat two more times.
5.add 100 ul of substrate/chromogen solution to each w
1、在微容器固定器中插入足够数量的容器，使所有标准品和样品能同样环境下运行。记录标准品位置和样品位置。
2、在每个相同的容器里加入50ul一份的标准溶液或预备品
3、在每个容器底部加入50ul稀释过的共轭酶，用手轻轻摇晃盘子以混合，室温下(20-25 C/68-77F)培育一小时。
4、从容器里的液体倒出，倒转微容器固定器在吸水纸上，用力拍打（一排拍三次）。确保容器里液体全部清除。然后用250ul的洗涤缓冲液（见10.1）注满所有容器，再倒出。重复2次以上。
5、在每个容器里加入100ul基质溶液或色原体溶液，用手轻轻摇晃盘子以混合。室温下（20-25C/68-77F）避光培育15分钟。
6、在每个容器里加入100ul中止液（一种生化试剂），用手轻轻摇晃盘子以混合。在450nm波长下，空白对照，测量吸光率。在加入中止液后的30分钟内读取结果。