UC知道生物专业英语翻译 能人帮忙啊
来源：百度知道 编辑：UC知道 时间：2024/07/06 23:53:45
Materials and methods
Reagent, strain, media and culture conditions
Anthraquinonic synthetic dye, RBBR, was purchased
from Sigma.
Trametes versicolor As 5.48 was obtained from
Chinese Academy of Sciences.
The Pichia methanolica strain used for heterologous
expression was PMAD11 (ade2-11). The
upstream primer and downstream were designed
based on the structural gene of Lcc1 (NCBI accession
no. X84683). The vector pMETaA (Invitrogen) was
used for expression in P. methanolica. The heterologous
expression of laccase from T. versicolor in
P. methanolica was performed as described (Guo
et al. 2005, 2006).
The laccase-secreting PMAD11 transformant was
inoculated into buffered dextrose complex medium
(BMDY; yeast extract 10 g l-1, peptone 20 g l-1, pH
6.0, 100 mM potassium phosphate buffer, yeast
nitrogen base (YNB) 13.4 g l-1, biotin 400 lg l-1,
dextrose 20 g l-1) and incubated at 30C with
Reagent, strain, media and culture conditions
Anthraquinonic synthetic dye, RBBR, was purchased
from Sigma.
Trametes versicolor As 5.48 was obtained from
Chinese Academy of Sciences.
The Pichia methanolica strain used for heterologous
expression was PMAD11 (ade2-11). The
upstream primer and downstream were designed
based on the structural gene of Lcc1 (NCBI accession
no. X84683). The vector pMETaA (Invitrogen) was
used for expression in P. methanolica. The heterologous
expression of laccase from T. versicolor in
P. methanolica was performed as described (Guo
et al. 2005, 2006).
The laccase-secreting PMAD11 transformant was
inoculated into buffered dextrose complex medium
(BMDY; yeast extract 10 g l-1, peptone 20 g l-1, pH
6.0, 100 mM potassium phosphate buffer, yeast
nitrogen base (YNB) 13.4 g l-1, biotin 400 lg l-1,
dextrose 20 g l-1) and incubated at 30C with
材料和方法
试剂，应变，媒体和文化条件
Anthraquinonic合成染料， RBBR ，购买
从西格玛。
变色栓菌作为获得5.48从
中国科学院院士。
在甲醇毕赤酵母株用于异种
表达PMAD11 （ ade2 - 11 ） 。那个
上游和下游引物设计
基于结构基因的Lcc1 （国立生物技术信息中心加入
否。 X84683 ） 。矢量pMETaA （盒）是
用于表达在体育甲醇。在异种
漆酶的表达从云芝中吨
体育甲醇进行描述（郭
等人。 2005年， 2006年） 。
该漆酶分泌转化为PMAD11
接种缓冲葡萄糖复杂介质
（ BMDY ;酵母提取物10克L - 1时，蛋白胨20克L - 1时， pH值
6.0 ， 100毫米钾磷酸盐缓冲液，酵母
氮基（ YNB ）十三点四克L - 1时，生物400 LG电子L - 1时，
20克葡萄糖1 - 1 ）和培养30 C的
握手（ 250转） 。当浑浊的文化
达到OD600 * 4 ，细胞的收获
离心在3000 9克为5分钟。细胞
悬浮在一个缓冲的甲醇复杂介质
（ BMMY ;酵母提取物10克L - 1时，蛋白胨20克L - 1时，
pH值6.5 100毫米钾磷酸盐缓冲液， YNB
一十三点四克L - 1时，生物400 LG电子L - 1时，甲醇8毫升1 - 1 ）
载有0.2毫米硫酸铜。该瓶孵育
在20 C和0.8 ％ （ V / V ）的甲醇添加dailySamples采取了间隔测定
漆酶的活动。
漆酶检测活动