翻译生物方面的英文摘要,急!!!

来源:百度知道 编辑:UC知道 时间:2024/07/03 04:12:55
本文在研究离体胚的胚的基础上,借助于均匀试验设计的方法,建立了新疆小叶白蜡愈伤组织诱导及继代培养、愈伤组织不定芽分化和腋芽分化与TDZ及NAA浓度的回归模型,进而建立并优化了组织培养体系。最终得到新疆小叶白蜡愈伤组织诱导的最佳外植体为子叶,在WPM+2.0 mg/L 6-BA + 1.0 mg/L NAA的培养基愈伤组织诱导率为83.33%,且黑暗处理10天可使诱导增大到93.33%;最佳诱导培养基为:WPM+0.11mg/L TDZ+1.46mg/L NAA,诱导率为98.03%;最佳愈伤组织继代培养基为:WPM+0.098mg/L TDZ+1.47mg/L NAA,增值倍数为6.04倍;最佳不定芽诱导培养基为:WPM+0.12mg/LTDZ+ 0.74~0.77mg/LNAA,其诱导率为68.3%;最佳茎段腋芽诱导培养基为:WPM+0.25mg/LTDZ+ 1.0mg/LNAA,腋芽诱导率为75.28%,平均腋芽数为4.5个,平均腋芽长为22.48mm。

In this paper, the study of embryos in vitro embryo on the basis of the experimental design through the use of uniform methods, the establishment of the Xinjiang Ericerus lobular callus induction and subculture, callus differentiation of adventitious buds and axillary bud differentiation and the concentration of TDZ and NAA return model, to establish and optimize the tissue culture system. Xinjiang was eventually Ericerus lobular best callus induction for cotyledon explants in WPM +2.0 mg / L 6-BA + 1.0 mg / L NAA medium for callus induction rate was 83.33%, and the dark for 10 days could increase to 93.33% induction; the best medium for induction: WPM +0.11 mg / L TDZ +1.46 mg / L NAA, induced rate of 98.03%; best callus subculture medium: WPM + 0.098mg / L TDZ +1.47 mg / L NAA, the value multiples of 6.04 times; the best medium for inducing adventitious buds: WPM +0.12 mg / LTDZ + 0.74 ~ 0.77mg/LNAA, the induction rate of 68.3%; the best stem paragraph for axillary bud induction m