repression domain SRDX---cres-t原理是什么呢?

CRES-T (chimeric repressor gene–silencing technology)

It dominantly suppresses the transcription of genes regulated by the transcription factor of interest and is a useful technique for functional analysis of transcription factors

For example, you can generate a CRES-T mutant library for transcription factors thta you are interested at, and use the library to identify the funcation/role of these transcription factors.

It involves only the expression of a fusion protein (a transcriptional activator with a repression domain). The repressive activity of the chimeric repressor can overcome the activities of endogenous and functionally redundant transcription factors, with resultant induction of a dominant negative phenotype.

I think this paper may help you

Hiratsu et al., 2003 K. Hiratsu, K. Matsui, T. Koyama and M. Ohme-Takagi, Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domai