UC知道翻译一段文摘：Process optimization for antifungal chitinase production
来源：百度知道 编辑：UC知道 时间：2024/07/06 15:31:03
Single-parameter optimization was carried out for the production of chitinase using a soil isolate belonging to Trichoderma harzianum in
solid-state fermentation. Maximum chitinase activity (3.18 U/gds) was obtained after 96 h of incubation at 30 ◦C when wheat bran moistened
(65.7%) with salt solution was supplemented with colloidal chitin (1%, w/w) and yeast extract (2%, w/w) used as the substrate. The inoculum
contains 4×107 spores of T. harzianum (TUBF 781). The growth of the fungus on wheat bran particle was visualized by a scanning electron
microscope. Chitinase activity was measured as the amount of N-acetyl glucosamine (NAG) liberated in mol/min under reaction conditions.
The crude extract showed antifungal activity against a wide range of fungal strains belonging to Aspergillus, Rhizopus and Mucor sp., and
was found significant against Aspergillus niger (NCIM 563).
谢
solid-state fermentation. Maximum chitinase activity (3.18 U/gds) was obtained after 96 h of incubation at 30 ◦C when wheat bran moistened
(65.7%) with salt solution was supplemented with colloidal chitin (1%, w/w) and yeast extract (2%, w/w) used as the substrate. The inoculum
contains 4×107 spores of T. harzianum (TUBF 781). The growth of the fungus on wheat bran particle was visualized by a scanning electron
microscope. Chitinase activity was measured as the amount of N-acetyl glucosamine (NAG) liberated in mol/min under reaction conditions.
The crude extract showed antifungal activity against a wide range of fungal strains belonging to Aspergillus, Rhizopus and Mucor sp., and
was found significant against Aspergillus niger (NCIM 563).
谢
唯一参量优化为chitinase的生产被执行了使用属于Trichoderma harzianum的土壤孤立固体发酵。 最大chitinase活动(3.18 U/gds)在96 h以后得到了孵出在30 ◦C，当麦子麸皮弄湿(65.7%)与盐水用胶质甲壳质(1%， w/w)和酵母抽提物(2%， w/w)补充了使用作为基体接种物包含4×107 T.孢子。 harzianum (TUBF 781)。 真菌的成长在麦子麸皮微粒由扫描电子显微镜形象化解放的mol或分钟在反应情况下， Chitinase活动被测量了，当数量在 N乙酰基氨基葡萄糖(老马)。粗暴萃取物显示了杀真菌剂的活动反对属于曲霉菌、黑霉和白霉属空间的大范围霉菌张力和被发现了重大反对天门皂脚(NCIM 563)。