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来源：百度知道 编辑：UC知道 时间：2024/07/04 05:38:27
Gene-specific oligonucleotide probes are currently used in microarrays to avoid cross-hybridization of highly similar sequences. We developed an approach to determine the optimal number and length of genespecific
probes for accurate transcriptional profiling studies. The study surveyed probe lengths from 25 to 1000 nt. Long probes yield better signal intensity thanshort probes. The signal intensity of short probes can be improved by addition of spacers or using higher probe ncentration for spotting. We also found that accurate gene expression measurement can be achieved with multiple probes per gene and fewer probes are needed if longer probes rather than shorter probes are used. Based on theoretical considerations that were confirmed experimentally, our results showed that 150mer is the optimal probe length for expression measurement. Gene-specific probes can be identified using a omputational approach for 150mer probes and they can be treated like long cDNA probes in terms of th
probes for accurate transcriptional profiling studies. The study surveyed probe lengths from 25 to 1000 nt. Long probes yield better signal intensity thanshort probes. The signal intensity of short probes can be improved by addition of spacers or using higher probe ncentration for spotting. We also found that accurate gene expression measurement can be achieved with multiple probes per gene and fewer probes are needed if longer probes rather than shorter probes are used. Based on theoretical considerations that were confirmed experimentally, our results showed that 150mer is the optimal probe length for expression measurement. Gene-specific probes can be identified using a omputational approach for 150mer probes and they can be treated like long cDNA probes in terms of th
基因特异性核酸探针,目前所使用的芯片,以避免交叉杂交高度相似序列. 我们开发了一种方法来确定最佳数量和长度genespecific探针准确transcriptional profiling研究. 这项调查探针长度从1925年至1000年台币. 长期的探索取得更好的信号强度thanshort探针. 信号强度的短探针,可提高除间隔或采用高探头ncentration进行检举. 我们还发现,精确的基因表达测量都能够实现与多种探针,每个基因少探头 如果需要较长的探针而非较短的探针使用. 基于理论考虑的是通过实验证实, 我们的结果显示150mer是最优的探头长度为表达测量. 基因特异性的探针,可确定用omputational办法150mer探针,他们可以被当作长期的cDNA 探针的杂交反应的高灵敏度检测. 我们的实验数据还显示,在探索中不产生良好的信号强度作出错误的表达比率的测量结果. 使用探针未经实验验证,该基因特异性探针150mer长度是必要的. 不过, 短寡核苷酸探针也做好基因表达分析,如果探针进行了实验验证,筛选或者 multiple probes per基因用于表达测量.