UC知道谁能帮忙翻译一下英文文献 -十万火急
来源：百度知道 编辑：UC知道 时间：2024/09/21 23:36:19
CMC-ase was assayed viscometrically as described by Maclachlan (1988) in a final volume of
1.0 mL containing 0.5% CMC (w/v) in 50 mM citrate, 50 mM phosphate, 0.1% (w/v) NaN3 adjusted
to the required pH, plus sufficient enzyme to evoke 5-20% loss in initial viscosity in 2 h at 32OC.
One unit of CMC-ase activity was defined as the amount of enzyme required to reduce viscosity by
1% in 2 h under the above conditions. The capacity of enzyme extracts to hydrolyse the backbone of
tamarind seed XG was measured under the same conditions as CMC-ase in the presence or absence of
0.02% (w/v) tamarind XG oligosaccharide. The activity is referred to here as XG-ase by analogy with
CMC-ase. This is not intended to imply that these enzyme activities are due necessarily to P-glucanases
which are specific to CMC or XG as substrates. Likewise, although one unit of XG-ase activity is
defined in the same way as one unit of CMC-ase activity, the absolute values
1.0 mL containing 0.5% CMC (w/v) in 50 mM citrate, 50 mM phosphate, 0.1% (w/v) NaN3 adjusted
to the required pH, plus sufficient enzyme to evoke 5-20% loss in initial viscosity in 2 h at 32OC.
One unit of CMC-ase activity was defined as the amount of enzyme required to reduce viscosity by
1% in 2 h under the above conditions. The capacity of enzyme extracts to hydrolyse the backbone of
tamarind seed XG was measured under the same conditions as CMC-ase in the presence or absence of
0.02% (w/v) tamarind XG oligosaccharide. The activity is referred to here as XG-ase by analogy with
CMC-ase. This is not intended to imply that these enzyme activities are due necessarily to P-glucanases
which are specific to CMC or XG as substrates. Likewise, although one unit of XG-ase activity is
defined in the same way as one unit of CMC-ase activity, the absolute values
我用谷歌翻译后如下：
中央军委酶活性测定viscometrically所描述的麦克拉克伦（ 1988年）在最后货量
一点○毫升含0.5 ％ ，中央军委（瓦特/ v ）在50毫米柠檬酸，磷酸50毫米， 0.1 ％ （瓦特/ V ）的nan3调整
到所需的pH值，再加上足够的酶，以唤起5-20 ％的损失初步粘度在2 h后在32oc 。
一个单位的羧甲基纤维素酶活性被界定为金额所需的酶，以减少粘度
1 ％ ，在2 h后，根据上述条件。的能力，酶提取物对hydrolyse骨干
罗望子种子xg测量相同的条件下羧甲基纤维素酶在的存在或缺席
0.02 ％ （瓦特/ V ）的罗望子xg低聚糖。该活动是指这里作为xg酶类推
羧甲基纤维素酶。这是无意暗示，这些酶活性是由于一定为P - glucanases
这是具体到管委会或xg作为衬底。同样地，虽然一个单位的xg -酶活性是
定义在相同的方式，作为一个单位的羧甲基纤维素酶活性，绝对的价值观，这些单位不应该
相比，因为相对的能力endohydrolysis的两个衬底上每摩尔酶
尚未确定。