UC知道医学文章翻译
来源：百度知道 编辑：UC知道 时间：2024/07/06 21:06:09
Safety study of an antimicrobial peptide lactocin 160, produced by the vaginal Lactobacillus rhamnosus.
OBJECTIVE: To evaluate the safety of the antimicrobial peptide, lactocin 160. METHODS: Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI) model was used for an in vivo safety evaluation. RESULTS: The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9). Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis). Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vagin
OBJECTIVE: To evaluate the safety of the antimicrobial peptide, lactocin 160. METHODS: Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI) model was used for an in vivo safety evaluation. RESULTS: The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9). Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis). Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vagin
安全性研究的一个抗菌肽lactocin 160 ，所产生的阴道鼠李糖乳杆菌。
目的：评价安全的抗菌肽， lactocin 160 。方法： Lactocin 160 ，一个产品的阴道益生菌鼠李糖乳杆菌160的毒性评价和刺激。体外人类器官阴道- ectocervical组织模型（ EpiVaginal ）采用的安全测试确定曝光时间，减少组织的活力50 ％ （血浆ET - 50 ） 。溶血活性的lactocin160测试用8 ％的人红细胞悬浮液。乳酸杆菌的敏感性，以lactocin160了研究。兔阴道刺激（ RVI ）模型，用于在体内的安全性评价。结果：血浆ET - 50的价值为17.5小时lactocin 160 （ 4.9小时，壬苯醇醚9 N9号） 。溶血活性的lactocin 160为8.2 ％ （ N9号共造成溶血） 。乳酸菌抵制高浓度的多肽准备。该模型显示RVI略有阴道刺激。平均刺激指数等级被评为“无” 。结论： Lactocin 160显示最小刺激和具有良好的应用潜力的阴道内。